De acordo com as mudanças mundiais ocorrendo atualmente , uma economia sustentável e baseada em biomassa pode ser considerada o futuro. O fundamento disso é a exploração máxima de diversas matérias primas disponíveis para produzir energia, combustíveis, materiais e comodidades com valor agregado dentro do conceito de biorefinaria. Uma forma de atingir este objetivo é o uso de solevntes ambientalmente aceitáveis. Este projeto interdisciplinar foca no uso de líquidos ionicos e fluidos supercríticos na conversão química e biotecnológica (com enzimas) da biomassa para uso subsequente dentro do conceito de biorefinaria. This interdisciplinary project aims to fully explore the potential of IONIC LIQUIDS and SUPERCRITICAL FLUIDS to burst the biorefinery concept. Ionic liquids (ILs) and supercritical fluids (scF) are versatile compounds that comply with green chemistry principles and that can greatly contribute to overcome the current scientific and technological hurdles that constrain the biorefinery to become a reality. To accomplish this challenge, this cutting-edge research project is devoted to the production of the 2nd generation biofuels and high value added products from lignocellulosic biomass. This project proposal is concretised in 3 interlinked tasks: Task1 . Synthesis of ILs – synthesis of tailor-made ionic liquid able to perform biomass pre-treatment and hydrolysis Task 2. Biomass pre-treatment with ILs and scCO2 – selective fractionation of various types of biomass to separate the main fractions – cellulose, hemicellulose and lignin. Task 3. Integrated biomass pre-treatment and hydrolysis with ILs – chemical one-pot biomass fractionation and hydrolysis with IL towards sugar monomers for the posterior bioconversion to energy and value added chemicals Task 4. Integrated biomass pre-treatment and hydrolysis with enzymes – biotechnological - enzymatic biomass pre-treatment and hydrolysis towards sugar monomers for the posterior bioconversion to energy and value added chemicals Task 5. Biomass enzymatic hydrolysis in scCO2 – The use of high pressure stable enzymes for the biomass hydrolysis in CO2 environment. The project presented in this call is a substantial extension of works executed in both collaborative projects existing in both institutions. This project aims to execute a parallel research line to the work proposed in the SUSTAINABLE SOLVENTS-ASSISTED TRANSFORMATION OF BIOMASS IN THE BIOREFINERY CONCEPT - PESQUISADOR VISTIANTE ESPECIAL project. It is also an extension and further development of the interesting results obtained in the frame of PROETHANOL 2G - INTEGRATION OF BIOLOGY AND ENGINEERING INTO AN ECONOMICAL AND ENERGY-EFFICIENT 2G BIOETHANOL project.